Extra-intestinal pathogenic Escherichia coli in the UK. The importance in bacteraemia versus urinary tract infection, colonisation of widespread clones and specific virulence factors
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Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and
bacteraemia in the UK and around the world. These E. coli primarily belong to phylogenetic groups B2
and D, with the clones ST131, ST127, ST95, ST73 and ST69 responsible for the majority of these
infections.
In the UK, studies of ExPEC have focused on isolates from the North of England, ST131 strains and ExPEC
that possess extended-spectrum beta-lactamase (ESBL) enzymes. Therefore, very little is understood
about the UK ExPEC population as a whole, the breadth of virulence factors contributing to these
infections and the differences between urinary and bloodstream-derived ExPEC.
In this study ST131 was more frequently detected in bloodstream isolates and ST95 was most prevalent
in urinary isolates. Comparative virulence of the major clones in the Galleria mellonella infection model
revealed ST131 isolates to effect the highest mortality, although serogroup O6, which is linked with
ST73, was also associated with high mortality, potentially explaining the success of ST73-O6 in
bacteraemia. Analysis of virulence factors identified pap, afa/dra and kpsMTII as important
determinants in isolates causing urosepsis and those of ST131, while fyuA and fimH were distinctly
lacking, demonstrating their role as colonisation factors rather than virulence factors.
Although these findings are important, with appropriate treatment of urinary tract infections
they can become redundant, as ExPEC would be eradicated before causing a severe infection such as
bacteraemia or urosepsis. In urinary isolates, resistance to trimethoprim approached 50% and ampicillin
resistance was >70%, while ST131 isolates as a whole demonstrated ciprofloxacin and trimethoprim
resistance >50%. Together these indicate that empirical UTI guidelines need to be revisited, to prevent
recurrence of infection and ascension to the kidneys and bloodstream. In addition, data from this study
can be used to develop a point-of-care test to detect ST131, to guide appropriate treatment, without
the delay associated with referring a urine specimen for microbiological investigation.
Authors
Ciesielczuk, HollyCollections
- Theses [3705]