The potential of neurofilaments analysis using dry-blood and plasma spots
dc.contributor.author | Lombardi, V | |
dc.contributor.author | Carassiti, D | |
dc.contributor.author | Giovannoni, G | |
dc.contributor.author | Lu, C-H | |
dc.contributor.author | Adiutori, R | |
dc.contributor.author | Malaspina, A | |
dc.date.accessioned | 2021-01-14T10:13:59Z | |
dc.date.available | 2019-11-04 | |
dc.date.available | 2021-01-14T10:13:59Z | |
dc.date.issued | 2020-01-09 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | ARTN 97 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/69700 | |
dc.description.abstract | The lack of biomarkers for an early diagnosis of neurodegenerative disorders (NDs) has hampered the development of therapeutics whose effect would be enhanced by a timely intervention. Neurofilaments light chain (Nf-L), an integral part of the axonal structure, has emerged as a robust fluid biomarker for fatal neurodegenerative disorders like amyotrophic lateral sclerosis (ALS). To facilitate large-scale studies into early-stage neurodegeneration, reduce costs of samples collection/processing and cold-chain storage, we describe the measurement of Nf-L in blood fractions obtained from dry blood spots (DBS) and dry plasma spots (DPS), two filter paper-based remote blood collection tools. To test the feasibility of using this approach, Nf-L analysis in DBS/DPS is compared to that in plasma obtained from the same blood sample, looking at Nf-L discriminatory power in the clinical stratification of ALS compared to healthy controls. With the best pre-analytical treatment for total protein recovery and using highly sensitive immunoassays, we report the detection of different Nf-L levels in DBS elute compared to reference plasma and DPS from the same blood samples. However, Nf-L measurement in DBS elutes provides a very good discrimination of ALS from healthy controls which is comparable to that obtained using plasma Nf-L assays. With the available immunodetection methods, we show that Nf-L measurement based on DPS microsampling is similar to that in plasma. The filter-paper biophysical characteristics and the interference of high haemoglobin concentration released by erythrocyte lysis is likely to perturb Nf-L detection in DBS elute. Further studies into DBS-based Nf-L detection and its analytical optimization are needed to make this method suitable for routine Nf-L blood analyses in neurodegeneration. | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.ispartof | SCIENTIFIC REPORTS | |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.title | The potential of neurofilaments analysis using dry-blood and plasma spots | en_US |
dc.type | Article | en_US |
dc.rights.holder | © 2020, The Author(s) | |
dc.identifier.doi | 10.1038/s41598-019-54310-y | |
pubs.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000517989900048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 10 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | A Multicentre Biomarker Resource Strategy in ALS: AMBRoSIA::MND Association | en_US |
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