Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly.
dc.contributor.author | Liu, Y | en_US |
dc.contributor.author | Gupta, GD | en_US |
dc.contributor.author | Barnabas, DD | en_US |
dc.contributor.author | Agircan, FG | en_US |
dc.contributor.author | Mehmood, S | en_US |
dc.contributor.author | Wu, D | en_US |
dc.contributor.author | Coyaud, E | en_US |
dc.contributor.author | Johnson, CM | en_US |
dc.contributor.author | McLaughlin, SH | en_US |
dc.contributor.author | Andreeva, A | en_US |
dc.contributor.author | Freund, SMV | en_US |
dc.contributor.author | Robinson, CV | en_US |
dc.contributor.author | Cheung, SWT | en_US |
dc.contributor.author | Raught, B | en_US |
dc.contributor.author | Pelletier, L | en_US |
dc.contributor.author | van Breugel, M | en_US |
dc.date.accessioned | 2020-10-23T08:46:28Z | |
dc.date.available | 2018-04-05 | en_US |
dc.date.issued | 2018-04-30 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/67740 | |
dc.description.abstract | Centrosomes are required for faithful chromosome segregation during mitosis. They are composed of a centriole pair that recruits and organizes the microtubule-nucleating pericentriolar material. Centriole duplication is tightly controlled in vivo and aberrations in this process are associated with several human diseases, including cancer and microcephaly. Although factors essential for centriole assembly, such as STIL and PLK4, have been identified, the underlying molecular mechanisms that drive this process are incompletely understood. Combining protein proximity mapping with high-resolution structural methods, we identify CEP85 as a centriole duplication factor that directly interacts with STIL through a highly conserved interaction interface involving a previously uncharacterised domain of STIL. Structure-guided mutational analyses in vivo demonstrate that this interaction is essential for efficient centriolar targeting of STIL, PLK4 activation and faithful daughter centriole assembly. Taken together, our results illuminate a molecular mechanism underpinning the spatiotemporal regulation of the early stages of centriole duplication. | en_US |
dc.format.extent | 1731 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Nat Commun | en_US |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject | Binding Sites | en_US |
dc.subject | Cell Line, Tumor | en_US |
dc.subject | Centrioles | en_US |
dc.subject | Chromosome Segregation | en_US |
dc.subject | Crystallography, X-Ray | en_US |
dc.subject | Cytoskeletal Proteins | en_US |
dc.subject | Gene Expression | en_US |
dc.subject | Humans | en_US |
dc.subject | Intracellular Signaling Peptides and Proteins | en_US |
dc.subject | Mitosis | en_US |
dc.subject | Models, Molecular | en_US |
dc.subject | Mutation | en_US |
dc.subject | Oncogene Proteins, Fusion | en_US |
dc.subject | Osteoblasts | en_US |
dc.subject | Protein Binding | en_US |
dc.subject | Protein Conformation, alpha-Helical | en_US |
dc.subject | Protein Conformation, beta-Strand | en_US |
dc.subject | Protein Interaction Domains and Motifs | en_US |
dc.subject | Protein-Serine-Threonine Kinases | en_US |
dc.subject | Recombinant Proteins | en_US |
dc.title | Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly. | en_US |
dc.type | Article | |
dc.rights.holder | © 2018 The Author(s) | |
dc.identifier.doi | 10.1038/s41467-018-04122-x | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29712910 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 9 | en_US |
dcterms.dateAccepted | 2018-04-05 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
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