CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions.
dc.contributor.author | Ochi, T | en_US |
dc.contributor.author | Quarantotti, V | en_US |
dc.contributor.author | Lin, H | en_US |
dc.contributor.author | Jullien, J | en_US |
dc.contributor.author | Rosa E Silva, I | en_US |
dc.contributor.author | Boselli, F | en_US |
dc.contributor.author | Barnabas, DD | en_US |
dc.contributor.author | Johnson, CM | en_US |
dc.contributor.author | McLaughlin, SH | en_US |
dc.contributor.author | Freund, SMV | en_US |
dc.contributor.author | Blackford, AN | en_US |
dc.contributor.author | Kimata, Y | en_US |
dc.contributor.author | Goldstein, RE | en_US |
dc.contributor.author | Jackson, SP | en_US |
dc.contributor.author | Blundell, TL | en_US |
dc.contributor.author | Dutcher, SK | en_US |
dc.contributor.author | Gergely, F | en_US |
dc.contributor.author | van Breugel, M | en_US |
dc.date.accessioned | 2020-10-23T08:40:32Z | |
dc.date.available | 2020-04-11 | en_US |
dc.date.issued | 2020-06-02 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/67738 | |
dc.description.abstract | Centrioles are cylindrical assemblies whose peripheral microtubule array displays a 9-fold rotational symmetry that is established by the scaffolding protein SAS6. Centriole symmetry can be broken by centriole-associated structures, such as the striated fibers in Chlamydomonas that are important for ciliary function. The conserved protein CCDC61/VFL3 is involved in this process, but its exact role is unclear. Here, we show that CCDC61 is a paralog of SAS6. Crystal structures of CCDC61 demonstrate that it contains two homodimerization interfaces that are similar to those found in SAS6, but result in the formation of linear filaments rather than rings. Furthermore, we show that CCDC61 binds microtubules and that residues involved in CCDC61 microtubule binding are important for ciliary function in Chlamydomonas. Together, our findings suggest that CCDC61 and SAS6 functionally diverged from a common ancestor while retaining the ability to scaffold the assembly of basal body-associated structures or centrioles, respectively. | en_US |
dc.format.extent | 674 - 689.e11 | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Structure | en_US |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject | CCDC61 | en_US |
dc.subject | Chlamydomonas | en_US |
dc.subject | SAS6 | en_US |
dc.subject | VFL3 | en_US |
dc.subject | XRCC4 | en_US |
dc.subject | basal body | en_US |
dc.subject | centriole | en_US |
dc.subject | centrosome | en_US |
dc.subject | cilia | en_US |
dc.subject | microtubule | en_US |
dc.subject | structural biology | en_US |
dc.title | CCDC61/VFL3 Is a Paralog of SAS6 and Promotes Ciliary Functions. | en_US |
dc.type | Article | |
dc.rights.holder | © 2020 The Author(s) | |
dc.identifier.doi | 10.1016/j.str.2020.04.010 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/32375023 | en_US |
pubs.issue | 6 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 28 | en_US |
dcterms.dateAccepted | 2020-04-11 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |
This item appears in the following Collection(s)
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.