| dc.contributor.author | Engl, C | en_US |
| dc.date.accessioned | 2020-06-04T09:57:17Z | |
| dc.date.available | 2020-04-24 | en_US |
| dc.date.available | 2020-06-04T09:57:17Z | |
| dc.date.issued | 2020-05-15 | en_US |
| dc.identifier.issn | 2041-1723 | en_US |
| dc.identifier.other | 2422 (2020) | en_US |
| dc.identifier.other | 2422 (2020) | en_US |
| dc.identifier.other | 2422 (2020) | en_US |
| dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/64647 | |
| dc.description.abstract | Transcription is fundamentally noisy, leading to significant heterogeneity across bacterial populations. Noise is often attributed to burstiness, but the underlying mechanisms and their dependence on the mode of promotor regulation remain unclear. Here, we measure E. coli single cell mRNA levels for two stress responses that depend on bacterial sigma factors with different mode of transcription initiation (σ70 and σ54). By fitting a stochastic model to the observed mRNA distributions, we show that the transition from low to high expression of the σ70-controlled stress response is regulated via the burst size, while that of the σ54-controlled stress response is regulated via the burst frequency. Therefore, transcription initiation involving σ54 differs from other bacterial systems, and yields bursting kinetics characteristic of eukaryotic systems. | en_US |
| dc.publisher | Nature Research (part of Springer Nature) | en_US |
| dc.relation.ispartof | Nature Communications | en_US |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
| dc.title | The route to transcription initiation determines the mode of transcriptional bursting in E. coli | en_US |
| dc.type | Article | |
| dc.rights.holder | © The Author(s) 2020 | |
| dc.identifier.doi | 10.1038/s41467-020-16367-6 | en_US |
| pubs.notes | Not known | en_US |
| pubs.publication-status | Published | en_US |
| pubs.volume | 11 | en_US |
| dcterms.dateAccepted | 2020-04-24 | en_US |
