• Login
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    Studies on the Programmable Protein Assembly via Genetically Encoded Native Chemical Ligation 
    •   QMRO Home
    • Queen Mary University of London Theses
    • Theses
    • Studies on the Programmable Protein Assembly via Genetically Encoded Native Chemical Ligation
    •   QMRO Home
    • Queen Mary University of London Theses
    • Theses
    • Studies on the Programmable Protein Assembly via Genetically Encoded Native Chemical Ligation
    ‌
    ‌

    Browse

    All of QMROCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    ‌
    ‌

    Administrators only

    Login
    ‌
    ‌

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Studies on the Programmable Protein Assembly via Genetically Encoded Native Chemical Ligation

    View/Open
    Wong_Wan Ling_PhD_final_011019.pdf (31.97Mb)
    Publisher
    Queen Mary University of London
    Metadata
    Show full item record
    Abstract
    Programmed protein assembly has vast potential in applications as diverse as bioreactors, smart materials and drug delivery. However, to realise this potential, exact control of the assembly process is required. Thus, this thesis describes generic genetically controlled methods to engineer the self-assembly of geometrically designed protein fusions to form user-defined structures. In particular, it shows how designed fusion proteins can be reacted to form fibres and encapsulations via split-intein mediated native chemical ligation in both one-pot and stepwise syntheses. When compatible fusions are mixed, they react quickly via a split-intein mediated native chemical ligation (NCL) to produce peptide bonded products in high yields (75 % yield). The correctly formed products can be purified to high homogeny and were shown to form the intended user-defined structures.
    Authors
    Wong, WL
    URI
    https://qmro.qmul.ac.uk/xmlui/handle/123456789/60639
    Collections
    • Theses [3919]
    Copyright statements
    The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author
    Twitter iconFollow QMUL on Twitter
    Twitter iconFollow QM Research
    Online on twitter
    Facebook iconLike us on Facebook
    • Site Map
    • Privacy and cookies
    • Disclaimer
    • Accessibility
    • Contacts
    • Intranet
    • Current students

    Modern Slavery Statement

    Queen Mary University of London
    Mile End Road
    London E1 4NS
    Tel: +44 (0)20 7882 5555

    © Queen Mary University of London.