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dc.contributor.authorPang, X
dc.contributor.authorLi, W
dc.contributor.authorLandwehr, E
dc.contributor.authorYuan, Y
dc.contributor.authorWang, W
dc.contributor.authorAzevedo, HS
dc.date.accessioned2019-09-04T10:34:06Z
dc.date.available2019-09-04T10:34:06Z
dc.date.issued2019-07-15
dc.identifier.citationPang, Xinqing et al. "Mimicking The Endothelial Glycocalyx Through The Supramolecular Presentation Of Hyaluronan On Patterned Surfaces". Faraday Discussions, 2019. Royal Society Of Chemistry (RSC), doi:10.1039/c9fd00015a. Accessed 4 Sept 2019.en_US
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/59500
dc.description.abstractThe glycocalyx is the immediate pericellular matrix that surrounds many cell types, including endothelial cells (ECs), and is typically composed of glycans (glycosaminoglycans, proteoglycans, and glycoproteins). The endothelial glycocalyx is rich in hyaluronic acid (HA), which plays an important role in the maintenance of vascular integrity, although fundamental questions about the precise molecular regulation mechanisms remain unanswered. Here, we investigate the contribution of HA to the regulation of endothelial function using model surfaces. The peptide sequence GAHWQFNALTVR, previously identified by phage display with strong binding affinity for HA and named Pep-1, was thiolated at the N-terminal to form self-assembled monolayers (SAMs) on gold (Au) substrates, and microcontact printing (μCP) was used to develop patterned surfaces for the controlled spatial presentation of HA. Acetylated Pep-1 and a scrambled sequence of Pep-1 were used as controls. The SAMs and HA-coated surfaces were characterized by X-ray photoelectron spectroscopy (XPS), contact angle measurements, and quartz crystal microbalance with dissipation (QCM-D) monitoring, which confirmed the binding and presence of thiolated peptides on the Au surfaces and the deposition of HA. Fluorescence microscopy showed the localization of fluorescently labelled HA only on areas printed with Pep-1 SAMs. Cell culture studies demonstrated that low molecular weight HA improved the adhesion of human umbilical vein endothelial cells (HUVECs) to the substrate and also stimulated their migration. This research provides insight into the use of SAMs for the controlled presentation of HA with defined size in cultures of HUVECs to study their functions.en_US
dc.languageeng
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofFaraday Discuss
dc.rightsThis is a pre-copyedited, author-produced version of an article accepted for publication in Faraday Discuss following peer review. The version of record is available https://pubs.rsc.org/en/content/articlelanding/2019/FD/C9FD00015A#!divAbstract
dc.titleMimicking the endothelial glycocalyx through the supramolecular presentation of hyaluronan on patterned surfaces.en_US
dc.typeArticleen_US
dc.rights.holder© The Royal Society of Chemistry 2019
dc.identifier.doi10.1039/c9fd00015a
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/31305856en_US
pubs.notesNot knownen_US
pubs.publication-statusPublished onlineen_US
rioxxterms.funderDefault funderen_US
rioxxterms.identifier.projectDefault projecten_US
rioxxterms.funder.projecteea3a0cf-7193-42f5-a8db-415e0e874d28en_US


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