Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour
dc.contributor.author | GAVARA, N | |
dc.contributor.author | Flores, L | |
dc.contributor.author | KEELING, M | |
dc.contributor.author | ZHANG, X | |
dc.contributor.author | SLIOGERYTE, K | |
dc.date.accessioned | 2019-03-18T13:57:08Z | |
dc.date.available | 2019-02-08 | |
dc.date.available | 2019-03-18T13:57:08Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Flores, L., Keeling, M., Zhang, X., Sliogeryte, K. and Gavara, N. (2019). Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour. Scientific Reports, [online] 9(1). Available at: https://www.nature.com/articles/s41598-019-40092-w [Accessed 18 Mar. 2019]. | en_US |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/56308 | |
dc.description.abstract | Live-imaging techniques are at the forefront of biology research to explore behaviour and function from sub-cellular to whole organism scales. These methods rely on intracellular fluorescent probes to label specific proteins, which are commonly assumed to only introduce artefacts at concentrations far-exceeding routine use. Lifeact, a small peptide with affinity for actin microfilaments has become a gold standard in live cell imaging of the cytoskeleton. Nevertheless, recent reports have raised concerns on Lifeact-associated artefacts at the molecular and whole organism level. We show here that Lifeact induces dose-response artefacts at the cellular level, impacting stress fibre dynamics and actin cytoskeleton architecture. These effects extend to the microtubule and intermediate filament networks as well as the nucleus, and ultimately lead to altered subcellular localization of YAP, reduced cell migration and abnormal mechanical properties. Our results suggest that reduced binding of cofilin to actin filaments may be the underlying cause of the observed Lifeact-induced cellular artefacts. | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.ispartof | Scientific Reports | |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.title | Lifeact-GFP alters F-actin organization, cellular morphology and biophysical behaviour | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2019 | |
dc.identifier.doi | 10.1038/s41598-019-40092-w | |
pubs.notes | Not known | en_US |
pubs.publication-status | Accepted | en_US |
dcterms.dateAccepted | 2019-02-08 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | CSK Fingerprints::European Commission | en_US |
qmul.funder | CSK Fingerprints::European Commission | en_US |
qmul.funder | CSK Fingerprints::European Commission | en_US |
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