dc.contributor.author | Greiller, CL | en_US |
dc.contributor.author | Suri, R | en_US |
dc.contributor.author | Jolliffe, DA | en_US |
dc.contributor.author | Kebadze, T | en_US |
dc.contributor.author | Hirsman, AG | en_US |
dc.contributor.author | Griffiths, CJ | en_US |
dc.contributor.author | Johnston, SL | en_US |
dc.contributor.author | Martineau, AR | en_US |
dc.date.accessioned | 2018-12-18T09:23:00Z | |
dc.date.available | 2018-11-23 | en_US |
dc.date.issued | 2018-11-23 | en_US |
dc.date.submitted | 2018-12-06T13:05:43.391Z | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/53863 | |
dc.description.abstract | Human rhinoviruses commonly cause upper respiratory infections, which may be complicated by secondary bacterial infection. Vitamin D replacement reduces risk of acute respiratory infections in vitamin D-deficient individuals, but the mechanisms by which such protection is mediated are incompletely understood. We therefore conducted experiments to characterise the influence of the major circulating metabolite 25-hydroxyvitamin D (25[OH]D) and the active metabolite 1,25-dihydroxyvitamin D (1,25[OH]2D) on responses of a respiratory epithelial cell line (A549 cells) to infection with a major group human rhinovirus (RV-16). Pre-treatment of A549 respiratory epithelial cells with a physiological concentration (10-7M) of 25(OH)D induced transient resistance to infection with RV-16 and attenuated RV-16-induced expression of the genes encoding intercellular adhesion molecule 1 (ICAM-1, a cell surface glycoprotein that acts as the cellular receptor for major group rhinoviruses) and platelet-activating factor receptor (PAFR, a G-protein coupled receptor implicated in adhesion of Streptococcus pneumoniae to respiratory epithelial cells). These effects were associated with enhanced expression of the genes encoding the NF-κB inhibitor IκBα and the antimicrobial peptide cathelicidin LL-37. Our findings suggest possible mechanisms by which vitamin D may enhance resistance to rhinovirus infection and reduce risk of secondary bacterial infection in vitamin D-deficient individuals. | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | J Steroid Biochem Mol Biol | en_US |
dc.rights | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | 1,25-dihydroxyvitamin D | en_US |
dc.subject | 25-hydroxyvitamin D | en_US |
dc.subject | CAMP | en_US |
dc.subject | Human rhinovirus | en_US |
dc.subject | ICAM1 | en_US |
dc.subject | IKBA | en_US |
dc.subject | PTAFR | en_US |
dc.subject | Respiratory epithelial cells | en_US |
dc.title | Vitamin D attenuates rhinovirus-induced expression of intercellular adhesion molecule-1 (ICAM-1) and platelet-activating factor receptor (PAFR) in respiratory epithelial cells. | en_US |
dc.type | Article | |
dc.identifier.doi | 10.1016/j.jsbmb.2018.11.013 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/30476590 | en_US |
pubs.notes | No embargo | en_US |
pubs.publication-status | Published online | en_US |
dcterms.dateAccepted | 2018-11-23 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |