dc.contributor.author | Antonelli, A | |
dc.contributor.author | Noort, WA | |
dc.contributor.author | Jaques, J | |
dc.contributor.author | de Boer, B | |
dc.contributor.author | de Jong-Korlaar, R | |
dc.contributor.author | Brouwers-Vos, AZ | |
dc.contributor.author | Lubbers-Aalders, L | |
dc.contributor.author | van Velzen, JF | |
dc.contributor.author | Bloem, AC | |
dc.contributor.author | Yuan, H | |
dc.contributor.author | de Bruijn, JD | |
dc.contributor.author | Ossenkoppele, GJ | |
dc.contributor.author | Martens, ACM | |
dc.contributor.author | Vellenga, E | |
dc.contributor.author | Groen, RWJ | |
dc.contributor.author | Schuringa, JJ | |
dc.date.accessioned | 2018-08-09T12:19:50Z | |
dc.date.available | 2018-08-09T12:19:50Z | |
dc.date.issued | 2016-12-22 | |
dc.date.submitted | 2017-02-25T10:25:28.234Z | |
dc.identifier.citation | Antonelli, A., et al. (2016). "Establishing human leukemia xenograft mouse models by implanting human bone marrow–like scaffold-based niches." Blood 128(25): 2949-2959. | en_US |
dc.identifier.issn | 0006-4971 | |
dc.identifier.other | 10.1182/blood-2016-05-719021 | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/43204 | |
dc.description | This research was originally published in Blood Online. Antonelli, A., et al.. Title. Blood. Prepublished December 22, 2016.; https://doi.org/10.1182/blood-2016-05-719021. | en_US |
dc.description.abstract | To begin to understand the mechanisms that regulate self-renewal, differentiation, and
transformation of human hematopoietic stem cells or to evaluate the efficacy of novel
treatment modalities, stem cells need to be studied in their own species-specific
microenvironment. By implanting ceramic scaffolds coated with human mesenchymal
stromal cells into immune-deficient mice, we were able to mimic the human bone marrow
niche. Thus, we have established a human leukemia xenograft mouse model in which a
large cohort of patient samples successfully engrafted, which covered all of the important
genetic and risk subgroups. We found that by providing a humanized environment, stem
cell self-renewal properties were better maintained as determined by serial transplantation
assays and genome-wide transcriptome studies, and less clonal drift was observed as
determined by exome sequencing. The human leukemia xenograft mouse models that we
have established here will serve as an excellent resource for future studies aimed at
exploring novel therapeutic approaches. (Blood. 2016;128(25):2949-2959) | en_US |
dc.description.sponsorship | This work was supported by Grant No. ERC-2011-StG 281474
from the European Research Council (J.J.S.) and by Grant No.
VU2011-5127 from the Dutch Cancer Foundation (R.W.J.G.). | en_US |
dc.format.extent | 2949 - 2959 | |
dc.language.iso | en | en_US |
dc.publisher | American Society of Hematology | en_US |
dc.relation.ispartof | BLOOD | |
dc.subject | human hematopoietic stem cells | en_US |
dc.subject | xenograft models | en_US |
dc.subject | leukemic stem cells | en_US |
dc.title | Establishing human leukemia xenograft mouse models by implanting human bone marrow-like scaffold-based niches | en_US |
dc.type | Article | en_US |
dc.rights.holder | The American Society of Hematology | |
pubs.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000392655100014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | |
pubs.declined | 2017-02-25T10:25:25.930+0000 | |
pubs.deleted | 2017-02-25T10:25:25.930+0000 | |
pubs.issue | 25 | |
pubs.publication-status | Published | |
pubs.volume | 128 | |