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dc.contributor.authorCalciolari, Een_US
dc.contributor.authorRavanetti, Fen_US
dc.contributor.authorStrange, Aen_US
dc.contributor.authorMardas, Nen_US
dc.contributor.authorBozec, Len_US
dc.contributor.authorCacchioli, Aen_US
dc.contributor.authorKostomitsopoulos, Nen_US
dc.contributor.authorDonos, Nen_US
dc.date.accessioned2018-07-16T09:16:49Z
dc.date.available2017-12-24en_US
dc.date.issued2018-06en_US
dc.date.submitted2018-02-19T16:29:32.067Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/42146
dc.description.abstractBACKGROUND AND OBJECTIVE: Although collagen membranes have been clinically applied for guided tissue/bone regeneration for more than 30 years, their in vivo degradation pattern has never been fully clarified. A better understanding of the different stages of in vivo degradation of collagen membranes is extremely important, considering that the biology of bone regeneration requires the presence of a stable and cell/tissue-occlusive barrier during the healing stages in order to ensure a predictable result. Therefore, the aim of this study was to investigate the degradation pattern of a porcine non-cross-linked collagen membrane in an in vivo model of guided bone regeneration (GBR). MATERIAL AND METHODS: Decalcified and paraffin-embedded specimens from calvarial defects of 18, 10-month-old Wistar rats were used. The defects were treated with a double layer of collagen membrane and a deproteinized bovine bone mineral particulate graft. At 7, 14 and 30 days of healing, qualitative evaluation with scanning electron microscopy and atomic force microscopy, and histomorphometric measurements were performed. Markers of collagenase activity and bone formation were investigated using an immunofluorescence technique. RESULTS: A significant reduction of membrane thickness was observed from 7 to 30 days of healing, which was associated with progressive loss of collagen alignment, increased collagen remodeling and progressive invasion of woven bone inside the membranes. A limited inflammatory infiltrate was observed at all time points of healing. CONCLUSION: The collagen membrane investigated was biocompatible and able to promote bone regeneration. However, pronounced signs of degradation were observed starting from day 30. Since successful regeneration is obtained only when cell occlusion and space maintenance exist for the healing time needed by the bone progenitor cells to repopulate the defect, the suitability of collagen membranes in cases where long-lasting barriers are needed needs to be further reviewed.en_US
dc.description.sponsorshipOsteology Foundation, Switzerland, through the Young Researcher Grant. Grant Number: 14‐061en_US
dc.format.extent430 - 439en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofJ Periodontal Resen_US
dc.rightsThis is the peer reviewed version of the following article: Calciolari, E., Ravanetti, F., Strange, A., Mardas, N., Bozec, L., Cacchioli, A., Kostomitsopoulos, N. and Donos, N. (2018). Degradation pattern of a porcine collagen membrane in an in vivo model of guided bone regeneration. Journal of Periodontal Research, 53(3), pp.430-439., which has been published in final form at https://doi.org/10.1111/jre.12530. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
dc.subjectcollagen membraneen_US
dc.subjectguided bone regenerationen_US
dc.subjecthistomorphometryen_US
dc.subjectimmunohistochemistryen_US
dc.subjectAnimalsen_US
dc.subjectBiocompatible Materialsen_US
dc.subjectBone Regenerationen_US
dc.subjectBone Transplantationen_US
dc.subjectCattleen_US
dc.subjectCollagenen_US
dc.subjectCollagenasesen_US
dc.subjectCytokinesen_US
dc.subjectGuided Tissue Regenerationen_US
dc.subjectInflammationen_US
dc.subjectMembranes, Artificialen_US
dc.subjectModels, Animalen_US
dc.subjectOsteogenesisen_US
dc.subjectRatsen_US
dc.subjectRats, Wistaren_US
dc.subjectTime Factorsen_US
dc.subjectWound Healingen_US
dc.titleDegradation pattern of a porcine collagen membrane in an in vivo model of guided bone regeneration.en_US
dc.typeArticle
dc.rights.holder© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
dc.identifier.doi10.1111/jre.12530en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29446096en_US
pubs.issue3en_US
pubs.notes12 monthsen_US
pubs.publication-statusPublisheden_US
pubs.volume53en_US
dcterms.dateAccepted2017-12-24en_US
qmul.funderDegradation of a porcine collagen membrane in a pre-clinical model of guided bone regeneration::Osteology Foundationen_US


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