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dc.contributor.authorHaugh, MGen_US
dc.contributor.authorThorpe, SDen_US
dc.contributor.authorVinardell, Ten_US
dc.contributor.authorBuckley, CTen_US
dc.contributor.authorKelly, DJen_US
dc.date.accessioned2018-03-14T12:08:11Z
dc.date.available2012-10-16en_US
dc.date.issued2012-12en_US
dc.date.submitted2018-03-12T16:42:50.528Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/35523
dc.description.abstractThe inherent biocompatibility of fibrin hydrogels makes them an attractive material for use in a wide range of tissue engineering applications. Despite this, their relatively low stiffness and high compliance limits their potential for certain orthopaedic applications. Enhanced mechanical properties are desirable so as to withstand surgical handling and in vivo loading after implantation and additionally, can provide important cues to cells seeded within the hydrogel. Standard methods used to enhance the mechanical properties of biological scaffolds such as chemical or thermal crosslinking cannot be used with fibrin hydrogels as cell seeding and gel formation occurs simultaneously. The objective of this study was to investigate the use of plastic compression as a means to improve the mechanical properties of chondrocyte-seeded fibrin hydrogels and to determine the influence of such compression on cell viability within these constructs. It was found that the application of 80% strain to fibrin hydrogels for 30 min (which resulted in a permanent strain of 47.4%) produced a 2.1-fold increase in the subsequent compressive modulus. Additionally, chondrocyte viability was maintained in the plastically compressed gels with significant cellular proliferation and extracellular matrix accumulation observed over 28 days of culture. In conclusion, plastic compression can be used to modulate the density and mechanical properties of cell-seeded fibrin hydrogels and represents a useful tool for both in theatre and in vitro tissue engineering applications.en_US
dc.format.extent66 - 72en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofJ Mech Behav Biomed Materen_US
dc.subjectAnimalsen_US
dc.subjectCartilageen_US
dc.subjectCell Survivalen_US
dc.subjectChondrocytesen_US
dc.subjectCompressive Strengthen_US
dc.subjectFeasibility Studiesen_US
dc.subjectFibrinen_US
dc.subjectHydrogelsen_US
dc.subjectMaterials Testingen_US
dc.subjectPlasticsen_US
dc.subjectSwineen_US
dc.subjectTime Factorsen_US
dc.titleThe application of plastic compression to modulate fibrin hydrogel mechanical properties.en_US
dc.typeArticle
dc.rights.holder© 2012 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.jmbbm.2012.10.009en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/23149099en_US
pubs.notesNot knownen_US
pubs.organisational-group/Queen Mary University of London
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering/Engineering and Materials Science - Staff
pubs.publication-statusPublisheden_US
pubs.volume16en_US
dcterms.dateAccepted2012-10-16en_US


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