dc.contributor.author | Perrin, RJ | en_US |
dc.contributor.author | Payton, JE | en_US |
dc.contributor.author | Barnett, DH | en_US |
dc.contributor.author | Wraight, CL | en_US |
dc.contributor.author | Woods, WS | en_US |
dc.contributor.author | Ye, L | en_US |
dc.contributor.author | George, JM | en_US |
dc.date.accessioned | 2012-10-19T08:57:36Z | |
dc.date.issued | 2003-10-02 | en_US |
dc.identifier.issn | 0304-3940 | en_US |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/2819 | |
dc.description.abstract | While alpha- and beta-synuclein largely overlap in their expression in the vertebrate brain, only alpha-synuclein accumulates in the fibrillar aggregates typical of Parkinson's disease. It is thus critical to have immunological reagents that distinguish between these two protein isoforms. The monoclonal antibody Syn-1 (Transduction Labs) has been frequently used for the specific detection of alpha-synuclein. In this report, the epitope for Syn-1 is localized within residues 91-99 of human alpha-synuclein. Sequence differences exist in this domain that account for the specificity of Syn-1 for alpha- versus beta-synuclein. However, Syn-1 also displays reactivity with additional species (approximately 45 kDa) in brain homogenates from both wild-type and alpha-synuclein null mice, indicating a potential for cross-reactivity with a protein species that is unrelated to alpha-synuclein in brain tissue or extracts. | en_US |
dc.format.extent | 133 - 135 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Neurosci Lett | en_US |
dc.subject | Animals | en_US |
dc.subject | Antibodies, Monoclonal | en_US |
dc.subject | Antibody Specificity | en_US |
dc.subject | Brain | en_US |
dc.subject | Cells, Cultured | en_US |
dc.subject | Epitope Mapping | en_US |
dc.subject | Humans | en_US |
dc.subject | Immunoblotting | en_US |
dc.subject | Mice | en_US |
dc.subject | Mice, Knockout | en_US |
dc.subject | Mutation | en_US |
dc.subject | Nerve Tissue Proteins | en_US |
dc.subject | Rats | en_US |
dc.subject | Recombinant Proteins | en_US |
dc.subject | Species Specificity | en_US |
dc.subject | Synucleins | en_US |
dc.subject | alpha-Synuclein | en_US |
dc.subject | beta-Synuclein | en_US |
dc.title | Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines. | en_US |
dc.type | Article | |
dc.rights.holder | Copyright © 2003 Elsevier Ireland Ltd. | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/12946570 | en_US |
pubs.issue | 2 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 349 | en_US |