dc.contributor.author | Subramanian, L | en_US |
dc.contributor.author | Toda, NRT | en_US |
dc.contributor.author | Rappsilber, J | en_US |
dc.contributor.author | Allshire, RC | en_US |
dc.date.accessioned | 2017-03-24T14:40:13Z | |
dc.date.issued | 2014-04-30 | en_US |
dc.date.submitted | 2017-03-01T08:32:32.855Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/22217 | |
dc.description.abstract | CENP-A chromatin forms the foundation for kinetochore assembly. Replication-independent incorporation of CENP-A at centromeres depends on its chaperone HJURP(Scm3), and Mis18 in vertebrates and fission yeast. The recruitment of Mis18 and HJURP(Scm3) to centromeres is cell cycle regulated. Vertebrate Mis18 associates with Mis18BP1(KNL2), which is critical for the recruitment of Mis18 and HJURP(Scm3). We identify two novel fission yeast Mis18-interacting proteins (Eic1 and Eic2), components of the Mis18 complex. Eic1 is essential to maintain Cnp1(CENP-A) at centromeres and is crucial for kinetochore integrity; Eic2 is dispensable. Eic1 also associates with Fta7(CENP-Q/Okp1), Cnl2(Nkp2) and Mal2(CENP-O/Mcm21), components of the constitutive CCAN/Mis6/Ctf19 complex. No Mis18BP1(KNL2) orthologue has been identified in fission yeast, consequently it remains unknown how the key Cnp1(CENP-A) loading factor Mis18 is recruited. Our findings suggest that Eic1 serves a function analogous to that of Mis18BP1(KNL2), thus representing the functional counterpart of Mis18BP1(KNL2) in fission yeast that connects with a module within the CCAN/Mis6/Ctf19 complex to allow the temporally regulated recruitment of the Mis18/Scm3(HJURP) Cnp1(CENP-A) loading factors. The novel interactions identified between CENP-A loading factors and the CCAN/Mis6/Ctf19 complex are likely to also contribute to CENP-A maintenance in other organisms. | en_US |
dc.description.sponsorship | L.S. was supported by an EC FP7 Marie Curie International Incoming Fellowship (PIIF-GA-2010-275280) and an EMBO Long Term Fellowship (ALTF 1491-2010). The Darwin Trust and a Principal's Career Development scholarship supported N.R.T.T. The Wellcome Trust supported the work of R.C.A. (095021 and 065061) and J.R. (084229) along with funding from the European Commission Network of Excellence EpiGeneSys (HEALTH-F4-2010-257082) to R.C.A. The Wellcome Trust Centre for Cell Biology (092076) and mass spectrometry instrumentation (091020) are supported by funding from the Wellcome Trust. R.C.A. is a Wellcome Trust Principal Research Fellow. | en_US |
dc.format.extent | 140043 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Open Biol | en_US |
dc.rights | Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. | |
dc.subject | CENP-A | en_US |
dc.subject | Mis18 | en_US |
dc.subject | centromeres | en_US |
dc.subject | epigenetics | en_US |
dc.subject | fission yeast | en_US |
dc.subject | Amino Acid Sequence | en_US |
dc.subject | Carrier Proteins | en_US |
dc.subject | Cell Cycle Proteins | en_US |
dc.subject | Centromere | en_US |
dc.subject | Chromatin | en_US |
dc.subject | Chromosomal Proteins, Non-Histone | en_US |
dc.subject | Cytoskeletal Proteins | en_US |
dc.subject | Histone Acetyltransferases | en_US |
dc.subject | Immunoprecipitation | en_US |
dc.subject | Kinetochores | en_US |
dc.subject | Molecular Sequence Data | en_US |
dc.subject | Mutation | en_US |
dc.subject | Protein Binding | en_US |
dc.subject | Saccharomyces cerevisiae Proteins | en_US |
dc.subject | Schizosaccharomyces | en_US |
dc.subject | Schizosaccharomyces pombe Proteins | en_US |
dc.subject | Sequence Alignment | en_US |
dc.title | Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. | en_US |
dc.type | Article | |
dc.rights.holder | © 2014 The Authors | |
dc.identifier.doi | 10.1098/rsob.140043 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/24789708 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 4 | en_US |