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    Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish. 
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    • Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish.
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    • Organismal Biology
    • Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish.
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    Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish.

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    Accepted version (1.168Mb)
    Volume
    143
    Pagination
    1464 - 1474
    DOI
    10.1242/dev.129155
    Journal
    Development
    Issue
    9
    Metadata
    Show full item record
    Abstract
    In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 h of a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. By contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Either mechanical lesions or motor neuron ablation is sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
    Authors
    Ohnmacht, J; Yang, Y; Maurer, GW; Barreiro-Iglesias, A; Tsarouchas, TM; Wehner, D; Sieger, D; Becker, CG; Becker, T
    URI
    http://qmro.qmul.ac.uk/xmlui/handle/123456789/15757
    Collections
    • Organismal Biology [244]
    Language
    eng
    Licence information
    Creative Commons Attribution License
    Copyright statements
    © 2016. The authors
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