dc.contributor.author | Lu, Zhengsun | |
dc.date.accessioned | 2016-09-07T12:27:29Z | |
dc.date.available | 2016-09-07T12:27:29Z | |
dc.date.issued | 2015-11-26 | |
dc.date.submitted | 2016-06-28T12:27:33.795Z | |
dc.identifier.citation | Lu, Z. 2015. Electrospun nanofiber scaffolds and crosslinked protein membranes as scaffold materials in tissue engineering. Queen Mary University of London | en_US |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/15023 | |
dc.description.abstract | Abstract
Scaffold materials play an essential role in tissue engineering field due to its function
of accommodate and guide cell proliferation. In this study, I investigated different
types of crosslinked protein membranes that can be produced in microfluidic channels
and a number of various types of PLGA electrospun composite nanofiber scaffold to
examine their potentials as scaffold materials in tissue engineering. A simplified
fabrication technique has been developed to produce a large surface area of crosslinked
protein membranes to fulfill the purpose of cell culture experiments. Bovine serum
albumin is used along with two acyl chloride crosslinkers, i.e. TCL and IDCL,
respectively to accomplish the cross-linking. On the other hand, PLGA is dissolved in
HFIP and enhanced with silk fibroin and carbon nanotubes to make composite
electrospun materials.
The morphology, physicochemical properties and biocompatibility of the membranes are studied. The biocompatibility of the membranes is investigated using cell proliferation of the PC12, ADSCs and neurons cultured on the
membranes. Our results show that compared to crosslinked protein membranes, the
electrospun materials are easier to prepare, less toxic and more suitable for mass
production. Moreover, the electrospun materials are seen to have better
biocompatibility in our cell culture study. Furthermore, the composite electrospun
materials with high CNTs concentrations demonstrate positive effects on the
proliferation of neurons | en_US |
dc.language.iso | en | en_US |
dc.publisher | Queen Mary University of London | en_US |
dc.subject | Materials Science | en_US |
dc.subject | Tissue Engineering | en_US |
dc.title | Electrospun nanofiber scaffolds and crosslinked protein membranes as scaffold materials in tissue engineering | en_US |
dc.type | Thesis | en_US |
dc.rights.holder | The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author | |