Validation of a Real-Time ISE Methodology to Quantify the Influence of Inhibitors of Demineralization Kinetics in vitro Using a Hydroxyapatite Model System.
dc.contributor.author | Huang, W-T | en_US |
dc.contributor.author | Shahid, S | en_US |
dc.contributor.author | Anderson, P | en_US |
dc.date.accessioned | 2018-06-22T15:50:33Z | |
dc.date.available | 2018-03-15 | en_US |
dc.date.issued | 2018 | en_US |
dc.date.submitted | 2018-05-22T14:47:38.623Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/40143 | |
dc.description.abstract | The aim was to validate a novel protocol to measure the cariostatic efficacies of demineralization inhibitors by repeating previous SMR (scanning microradiography) studies investigating the dose response of Zn2+ and F- on demineralization kinetics in vitro using real-time Ca2+ ion selective electrodes (ISEs). In this study, Ca2+ release was used as a proxy for the extent of demineralization. Forty-eight hydroxyapatite (HAP) discs were allocated into 16 groups (n = 3) and adding either increasing [Zn2+], or [F-], similar to those used in the previous SMR studies. Each HAP disc was immersed in 50 mL, pH 4.0, buffered acetic acid for 1 h, and real-time ISE methodology was used to monitor the rate of increase in [Ca2+] in the demineralization solution. Next, either zinc acetate or sodium fluoride was added into each demineralization solution accordingly. Then after each [Zn2+] or [F-] addition, the HAP disc was further demineralized for 1 h, and ISE measurements were continued. The percentage reduction in the rate of calcium loss from hydroxyapatite (PRCLHAP) at each [Zn2+] or [F-] was calculated from the decrease in Ca2+ release rate, similar to that used in the previous SMR studies. A log-linear relationship between mean PRCLHAP and inhibitor concentration was found for both Zn2+ and F-, similar to that reported for each ion in the previous SMR studies. In conclusion, real-time Ca2+ ISE systems can be used to measure the cariostatic efficacies of demineralization inhibitors. | en_US |
dc.format.extent | 598 - 603 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Caries Res | en_US |
dc.rights | ‘This is the peer-reviewed but unedited manuscript version of the following article: Huang WT, Shahid S, Anderson P. Validation of a Real-Time ISE Methodology to Quantify the Influence of Inhibitors of Demineralization Kinetics in vitro Using a Hydroxyapatite Model System. Caries research. 2018;52(6):598-603. The final, published version is available at https://doi.org/10.1159/000488597’ | |
dc.subject | Demineralization | en_US |
dc.subject | Dental caries | en_US |
dc.subject | Inhibitors | en_US |
dc.subject | Ion selective electrodes | en_US |
dc.subject | Real-time method | en_US |
dc.title | Validation of a Real-Time ISE Methodology to Quantify the Influence of Inhibitors of Demineralization Kinetics in vitro Using a Hydroxyapatite Model System. | en_US |
dc.type | Article | |
dc.identifier.doi | 10.1159/000488597 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29804111 | en_US |
pubs.issue | 6 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 52 | en_US |
dcterms.dateAccepted | 2018-03-15 | en_US |