Browsing by Author "Dunsby, C"
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Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.
Guo, W; Kumar, S; Görlitz, F; Garcia, E; Alexandrov, Y; Munro, I; Kelly, DJ; Warren, S; Thorpe, P; Dunsby, C (SAGE, 2019-01-10)We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore ... -
FLIM, FRET and high content analysis
Garcia, E; Guo, W; Kumar, S; Gorlitz, F; Sparks, H; Alexandrov, Y; Munro, I; Kelly, DJ; Warren, S; Chennell, G (SPIE, 2020-03)Fluorescence lifetime imaging (FLIM) provides a means to contrast different molecular species and to map variations in the local fluorophore molecular environment, including to read out Förster resonant energy transfer ...