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dc.contributor.authorKowalczyk, Aen_US
dc.contributor.authorEyice, Öen_US
dc.contributor.authorSchäfer, Hen_US
dc.contributor.authorPrice, ORen_US
dc.contributor.authorFinnegan, CJen_US
dc.contributor.authorvan Egmond, RAen_US
dc.contributor.authorShaw, LJen_US
dc.contributor.authorBarrett, Gen_US
dc.contributor.authorBending, GDen_US
dc.date.accessioned2015-12-17T16:15:50Z
dc.date.available2015-07-20en_US
dc.date.issued2015-10en_US
dc.date.submitted2015-11-19T11:05:43.940Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/9916
dc.description.abstractMicrobial degradation is a major determinant of the fate of pollutants in the environment. para-Nitrophenol (PNP) is an EPA-listed priority pollutant with a wide environmental distribution, but little is known about the microorganisms that degrade it in the environment. We studied the diversity of active PNP-degrading bacterial populations in river water using a novel functional marker approach coupled with [(13)C6]PNP stable isotope probing (SIP). Culturing together with culture-independent terminal restriction fragment length polymorphism analysis of 16S rRNA gene amplicons identified Pseudomonas syringae to be the major driver of PNP degradation in river water microcosms. This was confirmed by SIP-pyrosequencing of amplified 16S rRNA. Similarly, functional gene analysis showed that degradation followed the Gram-negative bacterial pathway and involved pnpA from Pseudomonas spp. However, analysis of maleylacetate reductase (encoded by mar), an enzyme common to late stages of both Gram-negative and Gram-positive bacterial PNP degradation pathways, identified a diverse assemblage of bacteria associated with PNP degradation, suggesting that mar has limited use as a specific marker of PNP biodegradation. Both the pnpA and mar genes were detected in a PNP-degrading isolate, P. syringae AKHD2, which was isolated from river water. Our results suggest that PNP-degrading cultures of Pseudomonas spp. are representative of environmental PNP-degrading populations.en_US
dc.description.sponsorshipWe thank Unilever and the Natural Environment Research Council (grant NE/J014168/1) for financial support.en_US
dc.format.extent6890 - 6900en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofAppl Environ Microbiolen_US
dc.rightshttp://dx.doi.org/10.1128/AEM.01794-15.
dc.rightshttp://dx.doi.org/10.1128/AEM.01794-15.
dc.subjectBacterial Proteinsen_US
dc.subjectBiodegradation, Environmentalen_US
dc.subjectCarbon Isotopesen_US
dc.subjectMolecular Sequence Dataen_US
dc.subjectNitrophenolsen_US
dc.subjectPhylogenyen_US
dc.subjectPseudomonasen_US
dc.subjectRNA, Ribosomal, 16Sen_US
dc.subjectRiversen_US
dc.titleCharacterization of para-Nitrophenol-Degrading Bacterial Communities in River Water by Using Functional Markers and Stable Isotope Probing.en_US
dc.typeArticle
dc.rights.holderCopyright © 2015, American Society for Microbiology.
dc.identifier.doi10.1128/AEM.01794-15en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/26209677en_US
pubs.issue19en_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume81en_US
dcterms.dateAccepted2015-07-20en_US


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