Cholangiocarcinoma cell lines: proteomic analysis and enhancing response to chemotherapy.
Abstract
Cholangiocarcinoma (CCA) is a rare cancer with a poor prognosis. Much of medical
research has focused on investigating cancers with a higher incidence and little focus
has been devoted to this disease.
The aim of this thesis was to perform a protein analysis of CCA and cholangiocyte cell
lines. Differences between immortalised cancer and normal cells were sought in
order to identify potential therapeutic targets and/or diagnostic tools.
A variety of CCA cell lines were used, reflecting both intra and extrahepatic disease.
The different subtypes of CCA through the developed and developing world are also
represented so differences were also sought between them. Proteomic analysis was
performed using DIGE with subsequent spot selection. Identified spots were
extracted and processed using mass spectrometry.
In addition, available chemotherapy agents were tested in vitro against the same cell
lines to check for their action and how this could be enhanced. A benzodiazepine
receptor antagonist (PK11195) was used to demonstrate apoptosis promotion in the
presence of established cytotoxic agents (gemcitabine, etoposide, 5 fluorouracil and
cisplatin). Cytotoxic assays were carried out using the SRB (Sulphorhodamine B)
assay. Cell lines were tested for benzodiazepine receptor status using qRTPCR and
response was correlated
Authors
Pericleous, StephanosCollections
- Theses [4364]