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dc.contributor.authorWong, WL
dc.date.accessioned2019-10-23T11:42:33Z
dc.date.issued01/10/2019
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/60639
dc.descriptionPhDen_US
dc.description.abstractProgrammed protein assembly has vast potential in applications as diverse as bioreactors, smart materials and drug delivery. However, to realise this potential, exact control of the assembly process is required. Thus, this thesis describes generic genetically controlled methods to engineer the self-assembly of geometrically designed protein fusions to form user-defined structures. In particular, it shows how designed fusion proteins can be reacted to form fibres and encapsulations via split-intein mediated native chemical ligation in both one-pot and stepwise syntheses. When compatible fusions are mixed, they react quickly via a split-intein mediated native chemical ligation (NCL) to produce peptide bonded products in high yields (75 % yield). The correctly formed products can be purified to high homogeny and were shown to form the intended user-defined structures.en_US
dc.language.isoenen_US
dc.publisherQueen Mary University of London
dc.subjectEconomics and Financeen_US
dc.subjectPolitical Economyen_US
dc.subjectInequalityen_US
dc.subjectBrazilen_US
dc.titleStudies on the Programmable Protein Assembly via Genetically Encoded Native Chemical Ligationen_US
dc.typeThesisen_US
dc.rights.holderThe copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author


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  • Theses [4099]
    Theses Awarded by Queen Mary University of London

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