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dc.contributor.authorThompson, CKen_US
dc.contributor.authorMeitzen, Jen_US
dc.contributor.authorReplogle, Ken_US
dc.contributor.authorDrnevich, Jen_US
dc.contributor.authorLent, KLen_US
dc.contributor.authorWissman, AMen_US
dc.contributor.authorFarin, FMen_US
dc.contributor.authorBammler, TKen_US
dc.contributor.authorBeyer, RPen_US
dc.contributor.authorClayton, DFen_US
dc.contributor.authorPerkel, DJen_US
dc.contributor.authorBrenowitz, EAen_US
dc.date.accessioned2014-01-23T14:36:23Z
dc.date.available2012-03-13en_US
dc.date.issued2012en_US
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/5344
dc.descriptionThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.description.abstractPhotoperiod and hormonal cues drive dramatic seasonal changes in structure and function of the avian song control system. Little is known, however, about the patterns of gene expression associated with seasonal changes. Here we address this issue by altering the hormonal and photoperiodic conditions in seasonally-breeding Gambel's white-crowned sparrows and extracting RNA from the telencephalic song control nuclei HVC and RA across multiple time points that capture different stages of growth and regression. We chose HVC and RA because while both nuclei change in volume across seasons, the cellular mechanisms underlying these changes differ. We thus hypothesized that different genes would be expressed between HVC and RA. We tested this by using the extracted RNA to perform a cDNA microarray hybridization developed by the SoNG initiative. We then validated these results using qRT-PCR. We found that 363 genes varied by more than 1.5 fold (>log(2) 0.585) in expression in HVC and/or RA. Supporting our hypothesis, only 59 of these 363 genes were found to vary in both nuclei, while 132 gene expression changes were HVC specific and 172 were RA specific. We then assigned many of these genes to functional categories relevant to the different mechanisms underlying seasonal change in HVC and RA, including neurogenesis, apoptosis, cell growth, dendrite arborization and axonal growth, angiogenesis, endocrinology, growth factors, and electrophysiology. This revealed categorical differences in the kinds of genes regulated in HVC and RA. These results show that different molecular programs underlie seasonal changes in HVC and RA, and that gene expression is time specific across different reproductive conditions. Our results provide insights into the complex molecular pathways that underlie adult neural plasticity.en_US
dc.format.extente35119 - ?en_US
dc.languageengen_US
dc.relation.ispartofPLoS Oneen_US
dc.subjectAnimalsen_US
dc.subjectBrainen_US
dc.subjectBreedingen_US
dc.subjectCluster Analysisen_US
dc.subjectGene Expression Profilingen_US
dc.subjectGene Expression Regulationen_US
dc.subjectHormonesen_US
dc.subjectMaleen_US
dc.subjectMolecular Sequence Annotationen_US
dc.subjectMolecular Sequence Dataen_US
dc.subjectReproducibility of Resultsen_US
dc.subjectSeasonsen_US
dc.subjectSparrowsen_US
dc.subjectTelencephalonen_US
dc.subjectVocalization, Animalen_US
dc.titleSeasonal changes in patterns of gene expression in avian song control brain regions.en_US
dc.typeArticle
dc.identifier.doi10.1371/journal.pone.0035119en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/22529977en_US
pubs.issue4en_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume7en_US
dcterms.dateAccepted2012-03-13en_US


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