Show simple item record

dc.contributor.authorSteward, AJen_US
dc.contributor.authorThorpe, SDen_US
dc.contributor.authorVinardell, Ten_US
dc.contributor.authorBuckley, CTen_US
dc.contributor.authorWagner, DRen_US
dc.contributor.authorKelly, DJen_US
dc.date.accessioned2018-04-05T13:44:44Z
dc.date.available2012-03-06en_US
dc.date.issued2012-07en_US
dc.date.submitted2018-03-12T17:19:35.457Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/36243
dc.description.abstractBoth hydrostatic pressure (HP) and cell-matrix interactions have independently been shown to regulate the chondrogenic differentiation of mesenchymal stem cells (MSCs). The objective of this study was to test the hypothesis that the response of MSCs to hydrostatic pressure will depend on the biomaterial within which the cells are encapsulated. Bone-marrow-derived MSCs were seeded into either agarose or fibrin hydrogels and exposed to 10 MPa of cyclic HP (1 Hz, 4 h per day, 5 days per week for 3 weeks) in the presence of either 1 or 10 ng ml(-1) of TGF-β3. Agarose hydrogels were found to support a spherical cellular morphology, while MSCs seeded into fibrin hydrogels attached and spread, with clear stress fiber formation. Hydrogel contraction was also observed in MSC-fibrin constructs. While agarose hydrogels better supported chondrogenesis of MSCs, HP only enhanced sulfated glycosaminoglycan (sGAG) accumulation in fibrin hydrogels, which correlated with a reduction in fibrin contraction. HP also reduced alkaline phosphatase activity in the media for both agarose and fibrin constructs, suggesting that this stimulus plays a role in the maintenance of the chondrogenic phenotype. This study demonstrates that a complex relationship exists between cell-matrix interactions and hydrostatic pressure, which plays a key role in regulating the chondrogenic differentiation of MSCs.en_US
dc.format.extent2153 - 2159en_US
dc.languageengen_US
dc.relation.ispartofActa Biomateren_US
dc.rightshttps://doi.org/10.1016/j.actbio.2012.03.016
dc.subjectAnimalsen_US
dc.subjectCell Proliferationen_US
dc.subjectCells, Cultureden_US
dc.subjectExtracellular Matrixen_US
dc.subjectFibrinen_US
dc.subjectImmunohistochemistryen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectMicroscopy, Confocalen_US
dc.subjectSepharoseen_US
dc.subjectSwineen_US
dc.titleCell-matrix interactions regulate mesenchymal stem cell response to hydrostatic pressure.en_US
dc.typeArticle
dc.rights.holderCopyright © 2012 Acta Materialia Inc. Published by Elsevier Ltd.
dc.identifier.doi10.1016/j.actbio.2012.03.016en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/22426136en_US
pubs.issue6en_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume8en_US
dcterms.dateAccepted2012-03-06en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record