Show simple item record

dc.contributor.authorObara, Ben_US
dc.contributor.authorRoberts, MAJen_US
dc.contributor.authorArmitage, JPen_US
dc.contributor.authorGrau, Ven_US
dc.date.accessioned2018-03-07T10:23:55Z
dc.date.available2013-02-11en_US
dc.date.issued2013-04-23en_US
dc.date.submitted2018-01-17T09:56:07.764Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/34365
dc.description.abstractBACKGROUND: Microscopy image segmentation lays the foundation for shape analysis, motion tracking, and classification of biological objects. Despite its importance, automated segmentation remains challenging for several widely used non-fluorescence, interference-based microscopy imaging modalities. For example in differential interference contrast microscopy which plays an important role in modern bacterial cell biology. Therefore, new revolutions in the field require the development of tools, technologies and work-flows to extract and exploit information from interference-based imaging data so as to achieve new fundamental biological insights and understanding. RESULTS: We have developed and evaluated a high-throughput image analysis and processing approach to detect and characterize bacterial cells and chemotaxis proteins. Its performance was evaluated using differential interference contrast and fluorescence microscopy images of Rhodobacter sphaeroides. CONCLUSIONS: Results demonstrate that the proposed approach provides a fast and robust method for detection and analysis of spatial relationship between bacterial cells and their chemotaxis proteins.en_US
dc.format.extent134 - ?en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofBMC Bioinformaticsen_US
dc.rightsCC-BY
dc.subjectBacteriaen_US
dc.subjectImage Processing, Computer-Assisteden_US
dc.subjectMicroscopy, Fluorescenceen_US
dc.subjectMicroscopy, Interferenceen_US
dc.subjectRhodobacter sphaeroidesen_US
dc.titleBacterial cell identification in differential interference contrast microscopy images.en_US
dc.typeArticle
dc.rights.holder© Obara et al.; licensee BioMed Central Ltd. 2013
dc.identifier.doi10.1186/1471-2105-14-134en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/23617824en_US
pubs.notesNot knownen_US
pubs.publication-statusPublished onlineen_US
pubs.volume14en_US
dcterms.dateAccepted2013-02-11en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record