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dc.contributor.authorMardakheh, FKen_US
dc.contributor.authorSailem, HZen_US
dc.contributor.authorKümper, Sen_US
dc.contributor.authorTape, CJen_US
dc.contributor.authorMcCully, RRen_US
dc.contributor.authorPaul, Aen_US
dc.contributor.authorAnjomani-Virmouni, Sen_US
dc.contributor.authorJørgensen, Cen_US
dc.contributor.authorPoulogiannis, Gen_US
dc.contributor.authorMarshall, CJen_US
dc.contributor.authorBakal, Cen_US
dc.date.accessioned2018-02-26T12:53:10Z
dc.date.available2016-11-01en_US
dc.date.issued2016-12-20en_US
dc.date.submitted2018-02-22T11:51:04.153Z
dc.identifier.other10.1039/C6MB00701E
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/33684
dc.description.abstractLocalisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.en_US
dc.description.sponsorship. CJM was a Gibb Life Fellow of CRUK. HZS and CB were funded by BBSRC project grant BB/J017183/1 and CRUK Programme Foundation Award (C37275/A20146). CJT is funded by a Sir Henry Wellcome Postdoctoral Fellowship (098847/Z/12/Z). CJ is funded by a Cancer Research UK Career Establishment Award (C37293/A12905) and a Cancer Research UK Institute Award (A19258).en_US
dc.format.extent92 - 105en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofMol Biosysten_US
dc.rightsCC-BY-NC
dc.subjectCell Fractionationen_US
dc.subjectCell Lineen_US
dc.subjectChromatography, Liquiden_US
dc.subjectCluster Analysisen_US
dc.subjectHumansen_US
dc.subjectIntracellular Spaceen_US
dc.subjectMass Spectrometryen_US
dc.subjectProtein Bindingen_US
dc.subjectProtein Interaction Mappingen_US
dc.subjectProtein Interaction Mapsen_US
dc.subjectProtein Transporten_US
dc.subjectProteomeen_US
dc.subjectProteomicsen_US
dc.subjectSensitivity and Specificityen_US
dc.subjectSubcellular Fractionsen_US
dc.titleProteomics profiling of interactome dynamics by colocalisation analysis (COLA).en_US
dc.typeArticle
dc.rights.holderThe Royal Society of Chemistry 2017
dc.identifier.doi10.1039/c6mb00701een_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27824369en_US
pubs.issue1en_US
pubs.notesNo embargoen_US
pubs.publication-statusPublisheden_US
pubs.volume13en_US


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