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dc.contributor.authorMandilaras, Konstantinos
dc.date.accessioned2013-01-11T14:05:37Z
dc.date.available2013-01-11T14:05:37Z
dc.date.issued2012
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/3156
dc.descriptionPhDen_US
dc.description.abstractHeme has been previously implicated in the function of the mammalian circadian clock but there has been no systematic investigation into the interplay between iron homeostasis and circadian rhythms. I have addressed this question in the model organism Drosophila melanogaster using two different approaches. First, I measured the amount and iron content of the iron storage protein ferritin, the activity of the iron sulfur cluster-containing enzyme aconitase and total heme content in whole bodies of Drosophila at different times during the 24-hour day-night cycle. I found no apparent fluctuation in these assays that would suggest circadian regulation of iron metabolism. Second, I tested whether RNA interference (RNAi) of iron homeostasis genes would alter the circadian behaviour of the flies. I selected 48 genes related to iron metabolism, silenced their expression specifically in cells expressing the circadian time-keeping transcription factor timeless and monitored the ability of these flies to sustain circadian rhythms in the absence of light cues. I found that when one of the two ferritin subunits, Ferritin 2 Light Chain Homologue (Fer2LCH) was silenced in clock cells the resulting flies displayed disrupted circadian rhythms in constant darkness. Expression of the circadian clock transcription factors timeless and period was disrupted in Fer2LCHRNAi flies. Inducing RNAi in restricted subsets of neurons I observed defects when Fer2LCH expression was reduced in the small ventral lateral neurons and in the dorsal lateral neurons that express the photoreceptor cryptochrome. An enhancer trap in Fer2LCH showed expression in a subset of these neurons. I propose a new role for Fer2LCH in the function of the biological clock in Drosophila melanogaster.en_US
dc.language.isoenen_US
dc.publisherQueen Mary University of London
dc.subjectMedicineen_US
dc.subjectHaemorrhagic shocken_US
dc.titleA screen for iron metabolism genes that influence circadian rhythms implicates Ferritin 2 Light Chain Homologue in the Drosophila clocken_US
dc.typeThesisen_US
dc.rights.holderThe copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author


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    Theses Awarded by Queen Mary University of London

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