dc.contributor.author | MILLER, Jacqueline | |
dc.date.accessioned | 2017-09-20T13:38:20Z | |
dc.date.available | 2017-09-20T13:38:20Z | |
dc.date.issued | 1983 | |
dc.date.submitted | 2017-09-20T14:07:36.627Z | |
dc.identifier.citation | Miller, J, 1983. CYANIDE METABOLISM IN BACTERIA AND PLANTS. Queen Mary University of London | en_US |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/25784 | |
dc.description | PhD | en_US |
dc.description.abstract | The metabolism of organic cyanides and amides was investigated
in a species of Rhodococcus newly isolated from garden soil for its
capacity to use acetonitrile as sole C and N source. Acetonitrilegrown
cells hydrolysed a number of nitriles and amides to ammonia;
although hydrogen cyanide CHCN1 may have also been hydrolysed thus,
the results were close to experimental uncertainty. The bacterium
was shown to utilise a variety of amides as the sole source of C and/or
N for growth although it could not grow with every corresponding nitrile.
Succinate/ammonium sulphate grown cells did not hydrolyse acetonitrile
or acetamide indicating that the enzymes involved in nitrile
degradation are subject to induction. Acetamide and acetate appeared
to be gratuitous inducers of the acetonitrilase; acetate also induced
the acetamidase.
Optimum conditions were sought for assaying the acetonitrilase
and acetamidase activities in whole bacteria. These enzyme activities
were also detected in cell-free extracts although the cells proved
difficult to disrupt. The nitrilase was found to be particularly thermolabile
whether assayed using whole bacteria or extracts. Freezing this
enzyme in the soluble form resulted in total loss of activity. The
amidase was more thermostableand cell-free extracts retained the ability
to hydrolyse acetamide after being frozen although the activity was
greatly reduced.
The metabolism of HCN was investigated further in a variety of
higher plants including both cyanogenic and non-cyanogenic species. The
enzymes investigated were 8-cyanoalanine synthase, rhodanese and formamide
hydro-lyase. $-Cyanoalanine synthase was found to be present in
every higher plant tested whereas rhodanese occurred far less
commonly. Formamide hydro-lyase activity was not detected in any of the
higher plants tested. Furthermore, a general trend was apparent between
the HCN-potential of each plant and cyanide metabolising activity, in
that the higher the HCN-potential, in general, the higher the cyanide
metabolising activity. | en_US |
dc.description.sponsorship | Westfield College Studentship followed by a University of London Postgraduate Studentship | en_US |
dc.language.iso | en | en_US |
dc.publisher | Queen Mary University of London | en_US |
dc.rights | The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author | |
dc.subject | organic cyanides | en_US |
dc.subject | Rhodococcus | en_US |
dc.title | CYANIDE METABOLISM IN BACTERIA AND PLANTS | en_US |
dc.type | Thesis | en_US |