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dc.contributor.authorBrugha, Ren_US
dc.contributor.authorLowe, Ren_US
dc.contributor.authorHenderson, AJen_US
dc.contributor.authorHolloway, JWen_US
dc.contributor.authorRakyan, Ven_US
dc.contributor.authorWozniak, Een_US
dc.contributor.authorMahmud, Nen_US
dc.contributor.authorSeymour, Ken_US
dc.contributor.authorGrigg, Jen_US
dc.contributor.authorShaheen, SOen_US
dc.date.accessioned2017-08-21T08:25:24Z
dc.date.available2017-08-15en_US
dc.date.issued2017-12en_US
dc.date.submitted2017-08-19T13:50:27.566Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/25323
dc.description.abstractAIM: Epidemiological studies of deoxyribonucleic acid (DNA) methylation in airway disease have largely been conducted using blood or buccal samples. However, given tissue specificity of DNA methylation, these surrogate tissues may not allow reliable inferences about methylation in the lung. We sought to compare the pattern of DNA methylation in blood, buccal and nasal epithelial cells to that in airway epithelial cells from children. METHODS: Samples of blood, and buccal, nasal and airway epithelium were obtained from six children undergoing elective anaesthesia for adenotonsillectomy. DNA methylation was assessed at 450 000 5'-C-phosphate-G-3' (CpG) sites using the Illumina HumanMethylation450 array. RESULTS: Eighteen samples from all sites were suitable for analysis. Hierarchical clustering demonstrated that the methylation profile in nasal epithelium was most representative of that in airway epithelium; the profile in buccal cells was moderately similar and that in blood was least similar. CONCLUSION: DNA methylation in blood poorly reflects methylation in airway epithelium. Future epidemiological studies of DNA methylation and airway diseases should consider measurement of methylation either in buccal cells or, preferably, in nasal epithelial cells.en_US
dc.description.sponsorshipAsthma UK, Barts and The London Charity.en_US
dc.format.extent2011 - 2016en_US
dc.languageengen_US
dc.language.isoenen_US
dc.relation.ispartofActa Paediatren_US
dc.rights"This is the peer reviewed version of the following article: Brugha, R., Lowe, R., John Henderson, A., Holloway, J. W., Rakyan, V., Wozniak, E., Mahmud, N., Seymour, K., Grigg, J. and Shaheen, S. O. , DNA methylation profiles between airway epithelium and proxy tissues in children. Acta Paediatr. doi:10.1111/apa.14027 which has been published in final form at https://doi.org/10.1111/apa.14027 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
dc.subjectAsthma and early wheezeen_US
dc.subjectEpigeneticsen_US
dc.subjectAdolescenten_US
dc.subjectBlood Cellsen_US
dc.subjectChilden_US
dc.subjectChild, Preschoolen_US
dc.subjectDNA Methylationen_US
dc.subjectEpithelial Cellsen_US
dc.subjectFemaleen_US
dc.subjectHumansen_US
dc.subjectMaleen_US
dc.subjectMouth Mucosaen_US
dc.subjectNasal Mucosaen_US
dc.subjectRespiratory Mucosaen_US
dc.titleDNA methylation profiles between airway epithelium and proxy tissues in children.en_US
dc.typeArticle
dc.identifier.doi10.1111/apa.14027en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/28833606en_US
pubs.issue12en_US
pubs.notesNot knownen_US
pubs.organisational-group/Queen Mary University of London
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/Blizard Institute
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/Blizard Institute/Centre for Primary Care and Public Health
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/Blizard Institute/Genomics and Child Health
pubs.organisational-group/Queen Mary University of London/REF
pubs.organisational-group/Queen Mary University of London/REF/REF - SMD - Blizard
pubs.organisational-group/Queen Mary University of London/REF/REF - UoA 01
pubs.organisational-group/Queen Mary University of London/REF/Ref - UoA 02
pubs.publication-statusPublisheden_US
pubs.volume106en_US
dcterms.dateAccepted2017-08-15en_US


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