Show simple item record

dc.contributor.authorWare, MA
dc.contributor.authorDall'Osto, L
dc.contributor.authorRuban, AV
dc.date.accessioned2017-05-18T14:09:49Z
dc.date.available2017-05-18T14:09:49Z
dc.date.issued2016
dc.date.submitted2017-05-16T11:44:28.777Z
dc.identifier.citationWare, Maxwell A., Luca Dall’Osto, and Alexander V. Ruban. "An In Vivo Quantitative Comparison Of Photoprotection In Arabidopsis Xanthophyll Mutants". Frontiers in Plant Science. N.p., 2017. Web. 18 May 2017.en_US
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/23165
dc.description.abstractContribution of different LHCII antenna carotenoids to protective NPQ (pNPQ) were tested using a range of xanthophyll biosynthesis mutants of Arabidopsis: plants were either devoid of lutein (lut2), violaxanthin (npq2), or synthesized a single xanthophyll species, namely violaxanthin (aba4npq1lut2), zeaxanthin (npq2lut2), or lutein (chy1chy2lut5). A novel pulse amplitude modulated (PAM) fluorescence analysis procedure, that used a gradually increasing actinic light intensity, allowed the efficiency of pNPQ to be tested using the photochemical quenching (qP) parameter measured in the dark (qPd). Furthermore, the yield of photosystem II (ΦPSII) was calculated, and the light intensity which induces photoinhibition in 50% of leaves for each mutant was ascertained. Photoprotective capacities of each xanthophyll were quantified, taking into account chlorophyll a/b ratios and excitation pressure. Here, light tolerance, pNPQ capacity, and ΦPSII were highest in wild type plants. Of the carotenoid mutants, lut2 (lutein-deficient) plants had the highest light tolerance, and the joint the highest ΦPSII with violaxanthin only plants. We conclude that all studied mutants possess pNPQ and a more complete composition of xanthophylls in their natural binding sites is the most important factor governing photoprotection, rather than any one specific xanthophyll suggesting a strong structural effect of the molecules upon the LHCII antenna organization and discuss the results significance for future crop development.en_US
dc.description.sponsorshipMW was supported by Queen Mary Principal’s research studentship and AR The Leverhulme Trust and UK Biotechnology and Biological Sciences Research Council. AR would like to acknowledge The Royal Society for the Wolfson Research Merit Awarden_US
dc.format.extent841 - ?
dc.languageeng
dc.publisherFrontiers Mediaen_US
dc.relation.ispartofFront Plant Sci
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.subjectArabidopsisen_US
dc.subjectnon-photochemical quenchingen_US
dc.subjectpNPQen_US
dc.subjectphotosystem IIen_US
dc.subjectxanthophyllsen_US
dc.titleAn In Vivo Quantitative Comparison of Photoprotection in Arabidopsis Xanthophyll Mutants.en_US
dc.typeArticleen_US
dc.rights.holderCopyright © 2016 Ware, Dall’Osto and Ruban
dc.identifier.doi10.3389/fpls.2016.00841
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27446097
pubs.organisational-group/Queen Mary University of London
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering/Biological and Chemical Sciences - Biology - Research Students
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering/Biological and Chemical Sciences - Staff
pubs.organisational-group/Queen Mary University of London/Faculty Reporting - Research Students
pubs.organisational-group/Queen Mary University of London/Faculty Reporting - Research Students/Faculty of Science & Engineering PGRs
pubs.publication-statusPublished online
pubs.volume7


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Return to top