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dc.contributor.authorAttwood, SJen_US
dc.contributor.authorCortes, Een_US
dc.contributor.authorHaining, AWMen_US
dc.contributor.authorRobinson, Ben_US
dc.contributor.authorLi, Den_US
dc.contributor.authorGautrot, Jen_US
dc.contributor.authorDel Río Hernández, Aen_US
dc.date.accessioned2016-12-05T14:57:13Z
dc.date.available2016-09-13en_US
dc.date.issued2016-09-30en_US
dc.date.submitted2016-12-03T15:12:00.199Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/18125
dc.description.abstractCells are known to respond to physical cues from their microenvironment such as matrix rigidity. Discrete adhesive ligands within flexible strands of fibronectin connect cell surface integrins to the broader extracellular matrix and are thought to mediate mechanosensing through the cytoskeleton-integrin-ECM linkage. We set out to determine if adhesive ligand tether length is another physical cue that cells can sense. Substrates were covalently modified with adhesive arginylglycylaspartic acid (RGD) ligands coupled with short (9.5 nm), medium (38.2 nm) and long (318 nm) length inert polyethylene glycol tethers. The size and length of focal adhesions of human foreskin fibroblasts gradually decreased from short to long tethers. Furthermore, we found cell adhesion varies in a linker length dependent manner with a remarkable 75% reduction in the density of cells on the surface and a 50% reduction in cell area between the shortest and longest linkers. We also report the interplay between RGD ligand concentration and tether length in determining cellular spread area. Our findings show that without varying substrate rigidity or ligand density, tether length alone can modulate cellular behaviour.en_US
dc.description.sponsorshipThis work was supported by the European Research Council to ADRH (grant agreement 282051). We wish to thank all CMBL members for help with this project.en_US
dc.format.extent34334 - ?en_US
dc.languageengen_US
dc.relation.ispartofSci Repen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
dc.rights© The Author(s) 2016
dc.titleAdhesive ligand tether length affects the size and length of focal adhesions and influences cell spreading and attachment.en_US
dc.typeArticle
dc.identifier.doi10.1038/srep34334en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27686622en_US
pubs.notesNo embargoen_US
pubs.publication-statusPublished onlineen_US
pubs.volume6en_US
dcterms.dateAccepted2016-09-13en_US


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