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dc.contributor.authorMariscal, Ven_US
dc.contributor.authorNürnberg, DJen_US
dc.contributor.authorHerrero, Aen_US
dc.contributor.authorMullineaux, CWen_US
dc.contributor.authorFlores, Een_US
dc.date.accessioned2016-11-21T16:22:33Z
dc.date.available2016-06-07en_US
dc.date.issued2016-09en_US
dc.date.submitted2016-11-17T10:14:08.779Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/17791
dc.description.abstractFilamentous, N2 -fixing, heterocyst-forming cyanobacteria grow as chains of cells that are connected by septal junctions. In the model organism Anabaena sp. strain PCC 7120, the septal protein SepJ is required for filament integrity, normal intercellular molecular exchange, heterocyst differentiation, and diazotrophic growth. An Anabaena strain overexpressing SepJ made wider septa between vegetative cells than the wild type, which correlated with a more spread location of SepJ in the septa as observed with a SepJ-GFP fusion, and contained an increased number of nanopores, the septal peptidoglycan perforations that likely accommodate septal junctions. The septa between heterocysts and vegetative cells, which are narrow in wild-type Anabaena, were notably enlarged in the SepJ-overexpressing mutant. Intercellular molecular exchange tested with fluorescent tracers was increased for the SepJ-overexpressing strain specifically in the case of calcein transfer between vegetative cells and heterocysts. These results support an association between calcein transfer, SepJ-related septal junctions, and septal peptidoglycan nanopores. Under nitrogen deprivation, the SepJ-overexpressing strain produced an increased number of contiguous heterocysts but a decreased percentage of total heterocysts. These effects were lost or altered in patS and hetN mutant backgrounds, supporting a role of SepJ in the intercellular transfer of regulatory signals for heterocyst differentiation.en_US
dc.description.sponsorshipPlan Nacional de Investigación, Spain, European Regional Development Fund. Grant Number: BFU2014-56757-P Queen Mary University of London College Studentship University of London Central Research Funden_US
dc.format.extent968 - 981en_US
dc.languageengen_US
dc.relation.ispartofMol Microbiolen_US
dc.rightsThis is a pre-copyedited, author-produced PDF of an article accepted for publication in Molecular Microbiology following peer review. The version of record is available http://onlinelibrary.wiley.com/doi/10.1111/mmi.13436/full
dc.subjectAnabaenaen_US
dc.subjectBacterial Proteinsen_US
dc.subjectDiffusionen_US
dc.subjectGene Expression Regulation, Bacterialen_US
dc.subjectPeptidoglycanen_US
dc.titleOverexpression of SepJ alters septal morphology and heterocyst pattern regulated by diffusible signals in Anabaena.en_US
dc.typeArticle
dc.rights.holder© 2016 John Wiley & Sons Ltd
dc.identifier.doi10.1111/mmi.13436en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27273832en_US
pubs.issue6en_US
pubs.notes12 monthsen_US
pubs.publication-statusPublisheden_US
pubs.volume101en_US


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