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dc.contributor.authorFitzgerald, AJ
dc.contributor.authorRai, PS
dc.contributor.authorMarchbank, T
dc.contributor.authorTaylor, GW
dc.contributor.authorGhosh, S
dc.contributor.authorRitz, BW
dc.contributor.authorPlayford, RJ
dc.date.accessioned2010-05-20T11:01:50Z
dc.date.available06-2005
dc.date.available2010-05-20T11:01:50Z
dc.date.issued2010-05-20T11:01:50Z
dc.identifier.issn0017-5749
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/155
dc.description.abstractBackground: A partially hydrolysed and dried product of pacific whiting fish is currently marketed as a health food supplement to support `` intestinal health''. However, there has been only limited scientific study regarding its true biological activity.Aims: We therefore tested its efficacy in a variety of models of epithelial injury and repair.Methods: Effects on proliferation were determined using [ H-3] thymidine incorporation into epithelial rat intestinal RIE- 1 and human colonic HT29 cells. Effects on restitution ( cell migration) were analysed using wounded HT29 monolayers and its ability to influence gastric injury analysed using a rat indomethacin restraint model. Partial characterisation of bioactive agents was performed using mass spectroscopy, high pressure liquid chromatography, and gas chromatography.Results: Both cell proliferation and cell migration were increased by about threefold when added at 1 mg/ ml ( p< 0.01). Gastric injury was reduced by 59% when gavaged at 25 mg/ ml ( p< 0.05), results similar to using the potent cytoprotective agent epidermal growth factor at 12.5 mg/ ml. The vast majority of biological activity was soluble in ethanol, with glutamine in its single, di-, and tripeptide forms probably accounting for approximately 40% of the total bioactivity seen. Fatty acid constituents may also have contributed to cell migratory activity.Conclusions: Fish protein hydrolysate possesses biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the injurious effects of non- steroidal anti- inflammatory drugs and other ulcerative conditions of the bowel. Further studies appear justified.
dc.format.extent775 - 781
dc.languageEN
dc.publisherB M J PUBLISHING GROUP
dc.relation.isreplacedby123456789/157
dc.relation.isreplacedbyhttps://qmro.qmul.ac.uk/jspui/handle/123456789/155
dc.subjectNONSTEROIDAL ANTIINFLAMMATORY DRUGS
dc.subjectDAMAGE
dc.subjectGUT
dc.subjectGLUTAMINE
dc.subjectILEUM
dc.titleReparative properties of a commercial fish protein hydrolysate preparation
dc.rights.holderCopyright © 2005 by Gut
dc.identifier.doi10.1136/gut.2004.060608
pubs.issue6
pubs.volume54


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