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dc.contributor.authorBangani, Nen_US
dc.contributor.authorNakiwala, Jen_US
dc.contributor.authorMartineau, ARen_US
dc.contributor.authorWilkinson, RJen_US
dc.contributor.authorWilkinson, KAen_US
dc.contributor.authorLowe, DMen_US
dc.date.accessioned2016-09-16T14:49:56Z
dc.date.available2016-05-23en_US
dc.date.issued2016-11-01en_US
dc.date.submitted2016-09-16T14:27:41.956Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/15391
dc.description.abstractUsing a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (±SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% ± 8.1%, P < 0.001) or anti-CD35 antibody (23.2% ± 10.6%, P < 0.05) versus anti-CD4 controls (29.9% ± 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1-uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis.en_US
dc.description.sponsorshipFunding: Wellcome Trust (104803, 097684, 087754, 084323); MRC (UK) U1175.02.002.00014; European Union (FP7 HEALTH-F3-2012-305578 and FP7-PEOPLE-2011-IRSES); National research Foundation of South Africa (96841).en_US
dc.format.extent263 - 267en_US
dc.languageengen_US
dc.relation.ispartofJ Acquir Immune Defic Syndren_US
dc.rightsThis final peer-reviewed manuscript is made available under the terms of the Creative Commons Attribution – Non-Commercial (CC BY- NC) license. The final published version can be found here: http://dx.doi.org/10.1097/QAI.0000000000001103
dc.subjectCD4 Lymphocyte Counten_US
dc.subjectFlow Cytometryen_US
dc.subjectGPI-Linked Proteinsen_US
dc.subjectHIV Infectionsen_US
dc.subjectHumansen_US
dc.subjectImmunologic Factorsen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectNeutrophilsen_US
dc.subjectOpsonin Proteinsen_US
dc.subjectPhagocytosisen_US
dc.subjectReceptors, Complement 3ben_US
dc.subjectReceptors, IgGen_US
dc.subjectTuberculosisen_US
dc.subjectViral Loaden_US
dc.titleBrief Report: HIV-1 Infection Impairs CD16 and CD35 Mediated Opsonophagocytosis of Mycobacterium tuberculosis by Human Neutrophils.en_US
dc.typeArticle
dc.rights.holderCopyright (c) 2016 Wolters Kluwer Health, Inc.
dc.identifier.doi10.1097/QAI.0000000000001103en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27258232en_US
pubs.issue3en_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume73en_US


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