|dc.contributor.author||Howlett, Julie Antoinette||
|dc.description.abstract||A sequential study of the invasion of oral mucosa by C. albicans is
difficult to accomplish in human beings or in animal models. An in vitro
model of oral candidal infection has therefore been established and
evaluated by light and electron microscopy.
In the model system cultured oral mucosa from neonatal rats and
rabbits was infected with C. albicans. The pattern of invasion of the
tissues was similar to that reported in vivo although invasion tended to
be more extensive and progressed to the underlying connective tissues.
The system was evaluated by comparing the invasion of keratinized
and non-keratinized epithelium by a number of Candida species; their
ability to invade oral mucosa was in accord with their differing pathogenicities,
and the extent of invasion of the epithelium by the less
pathogenic species was related to its degree of keratinization.
The ultrastructural relationship between the superficial epithelium
and the fungi was similar to that seen in human oral candidosis, thus
validating the model as one in which to study the invasion of epithelium
by C. albicans. In examining the fine structure of the invading fungi
changes were observed in the organization of the fungal cell wall during
the invasive process. Cytochemical localization of acid phosphatase and
phospholipase demonstrated that these enzymes are located on the surface
of Candida within the tissues although their role in epithelial cell
membrane penetration was not clearly established. It is suggested that
both fungal enzymes and mechanical force may facilitate fungal invasion.
The deep candidal invasion seen in culture did not result from
changes within the epithelium but probably reflected the lack of systemic
factors. ' An evaluation of the role of immune and non-immune serum factors
in the model system indicated that these factors alone may not restrict
candidal penetration in vivo.||
|dc.title||An experimental study of the infection of oral mucosa in vitro by candida.||en_US
|dc.rights.holder||The copyright of this thesis rests with the author and no quotation from it or information derived from it may be published without the prior written consent of the author||