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    The Spectrin cytoskeleton regulates the Hippo signalling pathway. 
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    The Spectrin cytoskeleton regulates the Hippo signalling pathway.

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    Published version (8.515Mb)
    Volume
    34
    Pagination
    940 - 954
    DOI
    10.15252/embj.201489642
    Journal
    EMBO J
    Issue
    7
    Metadata
    Show full item record
    Abstract
    The Spectrin cytoskeleton is known to be polarised in epithelial cells, yet its role remains poorly understood. Here, we show that the Spectrin cytoskeleton controls Hippo signalling. In the developing Drosophila wing and eye, loss of apical Spectrins (alpha/beta-heavy dimers) produces tissue overgrowth and mis-regulation of Hippo target genes, similar to loss of Crumbs (Crb) or the FERM-domain protein Expanded (Ex). Apical beta-heavy Spectrin binds to Ex and co-localises with it at the apical membrane to antagonise Yki activity. Interestingly, in both the ovarian follicular epithelium and intestinal epithelium of Drosophila, apical Spectrins and Crb are dispensable for repression of Yki, while basolateral Spectrins (alpha/beta dimers) are essential. Finally, the Spectrin cytoskeleton is required to regulate the localisation of the Hippo pathway effector YAP in response to cell density human epithelial cells. Our findings identify both apical and basolateral Spectrins as regulators of Hippo signalling and suggest Spectrins as potential mechanosensors.
    Authors
    Fletcher, GC; Elbediwy, A; Khanal, I; Ribeiro, PS; Tapon, N; Thompson, BJ
    URI
    http://qmro.qmul.ac.uk/xmlui/handle/123456789/11898
    Collections
    • Centre for Tumour Biology [306]
    Language
    eng
    Licence information
    CC-BY Published under the terms of the CC BY 4.0 license
    Copyright statements
    © 2015 The Authors.

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